Sandwich ELISA- Steps and Advantages


ELISA stands for enzyme-linked immunosorbent assay. It is a widely used technique in biomedical research and clinical diagnostics that uses antibodies and enzymes to detect and quantify specific molecules in a sample. ELISA is based on the principle of antigen-antibody binding, which is a specific and selective interaction between two molecules that have complementary shapes and charges. Antibodies are proteins produced by the immune system that can recognize and bind to foreign molecules (antigens) with high specificity and affinity. Enzymes are biological catalysts that can speed up chemical reactions and produce a measurable signal, such as a color change or a fluorescent glow.