Ring Precipitation Test- Principle, Procedure, Results, Examples


Ring precipitation test is a simple and rapid method to detect the presence and quantity of antigen-antibody complexes in a solution. Antigen-antibody complexes are formed when specific antibodies bind to their corresponding antigens, which are molecules that can elicit an immune response. These complexes can precipitate out of solution when they reach an optimal ratio of antigen to antibody, forming a visible ring at the interface of two liquid layers.

Ring precipitation test has several objectives and applications in immunology and clinical diagnosis. Some of the main objectives are:

  • To detect antigen-antibody complexes in a solution and determine their relative concentration by comparing the size and intensity of the ring formation.
  • To identify the type and specificity of antibodies in a serum sample by using known antigens as test reagents.
  • To diagnose certain diseases or infections by detecting specific antibodies or antigens in the patient`s serum or body fluids.