Polyacrylamide Gel Electrophoresis (PAGE)


Polyacrylamide gel electrophoresis (PAGE) is a technique that allows the separation and analysis of proteins and nucleic acids based on their size and charge. PAGE can be performed under denaturing or native conditions, depending on the purpose of the experiment. Denaturing conditions disrupt the secondary and tertiary structure of the biomolecules, making them linear and uniformly charged. Native conditions preserve the natural conformation and charge of the biomolecules, allowing the separation based on both size and shape.