Microdase (Modified Oxidase) Test- Principle, Procedure, Results


The microdase test is a simple and rapid method to differentiate gram-positive, catalase-positive cocci (micrococci from staphylococci). Gram-positive cocci are spherical bacteria that stain purple with the Gram stain and produce bubbles when exposed to hydrogen peroxide (catalase test). However, these characteristics are not enough to distinguish between the genera Micrococcus and Staphylococcus, which have different clinical and epidemiological significance. Micrococci are generally harmless commensals of the skin and mucous membranes, while staphylococci are opportunistic pathogens that can cause a variety of infections, such as skin infections, food poisoning, endocarditis, and toxic shock syndrome. Therefore, it is important to identify these bacteria accurately in the laboratory.