Hippurate Hydrolysis Test- Principle, Procedure, Result, Uses


Hippurate hydrolysis test is a biochemical test that differentiates microorganisms on the basis of their ability to hydrolyze hippurate into glycine and benzoic acid by the enzymatic action of hippuricase. Hippurate is a salt or ester of hippuric acid, which is a compound formed by the conjugation of benzoic acid and glycine in the liver and excreted in urine. Some bacteria can break down hippurate by producing an enzyme called hippuricase, which cleaves the bond between benzoic acid and glycine. The resulting products are then detected by different indicators, such as ferric chloride or ninhydrin.