Esculin Hydrolysis Test- Principle, Procedure, Results, Uses


Esculin hydrolysis test is a biochemical test that can be used to differentiate bacteria based on their ability to hydrolyze esculin, a compound derived from the horse chestnut tree (Aesculus hippocastanum). Esculin hydrolysis is a common characteristic of many bacteria, including gram-positive and gram-negative bacteria, aerobes, facultative anaerobes, and anaerobes. The test can be used as a taxonomic tool in the identification of various microorganisms, such as Enterobacteriaceae, Streptococcus, Listeria, Aeromonas, and others.