Anthrone Test- Definition, Principle, Procedure, Result, Uses


Anthrone test is a group test for carbohydrates that provides a rapid and convenient method for quantification of carbohydrates that are either free or bound to any lipids or proteins. Carbohydrates are organic compounds that consist of carbon, hydrogen and oxygen atoms in a fixed ratio. They are the main source of energy for living organisms and are involved in various biological processes. Carbohydrates can be classified into different types based on their size and structure, such as monosaccharides, disaccharides, oligosaccharides and polysaccharides. Monosaccharides are the simplest and smallest carbohydrates that cannot be hydrolyzed into smaller units. Examples of monosaccharides are glucose, fructose and galactose. Disaccharides are composed of two monosaccharide units linked by a glycosidic bond. Examples of disaccharides are sucrose, lactose and maltose. Oligosaccharides are composed of 3 to 10 monosaccharide units linked by glycosidic bonds. Examples of oligosaccharides are raffinose, stachyose and fructooligosaccharides. Polysaccharides are composed of more than 10 monosaccharide units linked by glycosidic bonds. Examples of polysaccharides are starch, glycogen, cellulose and chitin.

Some carbohydrates are free in solution, while others are bound to lipids or proteins to form complex molecules such as glycoproteins and glycolipids. Glycoproteins are proteins that have one or more carbohydrate chains attached to them. They play important roles in cell recognition, signaling, adhesion and immunity. Glycolipids are lipids that have one or more carbohydrate chains attached to them. They are mainly found in the cell membrane and function as receptors, antigens and markers.

Anthrone test is a chemical test that can detect and measure the amount of carbohydrates in a given solution, regardless of whether they are free or bound to lipids or proteins. Anthrone test is based on the reaction of carbohydrates with anthrone reagent in the presence of concentrated sulfuric acid. Anthrone reagent is a yellow crystalline compound that contains naphthol as its main component. Anthrone test can be used for both qualitative and quantitative analysis of carbohydrates in various samples such as blood serum, milk, urine, plant extracts and food products.