Decarboxylase Test- Principle, Procedure, Results, Uses


Decarboxylase test is a biochemical test that determines the ability of bacteria to produce decarboxylase enzymes that break down amino acids. Decarboxylase enzymes are important for the metabolism of amino acids in bacteria, and they also play a role in the production of various biogenic amines that have physiological effects on humans and animals. Decarboxylase test is mainly used to differentiate members of the Enterobacteriaceae family, which are gram-negative rods that inhabit the intestinal tract of humans and animals. Some members of this family are pathogenic and cause diseases such as typhoid fever, dysentery, and urinary tract infections. Decarboxylase test can also be used to identify other bacteria that produce decarboxylase enzymes, such as Vibrio, Plesiomonas, Aeromonas, Stenotrophomonas, Burkholderia, Pseudomonas, and Streptococcus.