Rocket Immunoelectrophoresis- Objectives, Principle, Procedure, Results, Uses


Rocket immunoelectrophoresis is a laboratory technique that allows the quantification of antigens in a sample by measuring the height of a precipitin peak formed by the antigen-antibody reaction. The technique is based on the principle of electrophoresis, which is the movement of charged particles in an electric field. In rocket immunoelectrophoresis, the antigen is negatively charged and migrates towards the positive electrode, while the antibody is immobilized in the gel matrix. As the antigen encounters the antibody, it forms a visible immune complex that resembles a rocket. The height of the rocket is proportional to the concentration of the antigen in the sample.

Rocket immunoelectrophoresis was first described by Laurell in 1966 as a method for quantifying serum proteins. Since then, it has been widely used for various applications in clinical and research settings, such as detecting and measuring immunoglobulins, enzymes, hormones, toxins, viruses, and bacteria. Rocket immunoelectrophoresis is also known as electroimmunodiffusion, immunoelectrophoretic assay, or Laurell`s method.