Mucic Acid Test- Definition, Principle, Procedure, Result, Uses


Mucic acid test is a biochemical test that is used to detect the presence of galactose or lactose in a given sample. Galactose and lactose are carbohydrates that have a six-carbon sugar ring with an aldehyde group at one end and a primary alcohol group at the other end. These functional groups can be oxidized by a strong oxidizing agent like nitric acid to form dicarboxylic acids, which are also called saccharic acids. Mucic acid is one such saccharic acid that is formed from the oxidation of galactose or lactose. Mucic acid is insoluble in water and forms white or yellowish crystals that can be seen at the bottom of the test tube. The mucic acid test is highly specific for galactose and lactose and can distinguish them from other sugars that do not form insoluble saccharic acids. The mucic acid test is also known as galactaric acid test, because mucic acid is also called galactaric acid. The mucic acid test is a simple and reliable method to identify galactose or lactose in food samples, synthetic materials, and biological specimens.