Kirby Bauer Disc Diffusion Method For Antibiotic Susceptibility Testing


The aim of the Kirby Bauer disc diffusion method is to determine the antibiotics of choice to treat an infection and monitor antimicrobial resistance. Antimicrobial resistance is the ability of microorganisms to withstand the effects of antibiotics and other antimicrobial agents. It poses a serious threat to public health and can compromise the effectiveness of treatments for various infections.

The Kirby Bauer disc diffusion method is a simple and widely used technique to test the susceptibility of bacteria to different antibiotics. It involves placing antibiotic discs on an agar plate that has been inoculated with a bacterial culture. The antibiotics diffuse from the discs into the agar and create zones of inhibition around them, where the bacterial growth is inhibited. The size of the zones of inhibition indicates the level of susceptibility or resistance of the bacteria to the antibiotics.

By measuring the zones of inhibition and comparing them with standard guidelines, one can determine whether the bacteria are susceptible (S), intermediate (I), or resistant (R) to the antibiotics. This information can help clinicians to choose the most appropriate antibiotic for treating an infection and avoid using ineffective or unnecessary antibiotics that may contribute to antimicrobial resistance.

The Kirby Bauer disc diffusion method can also be used to monitor the trends and patterns of antimicrobial resistance among different bacteria and regions. This can help to identify emerging or resistant strains of bacteria and inform public health policies and interventions.