KIA Test- Principle, Media, Procedure, Results, Uses


Kligler’s Iron Agar (KIA) test is a differential medium that is used to identify and differentiate Gram-negative enteric bacilli based on their ability to ferment glucose and lactose and produce hydrogen sulfide (H2S) gas. It is one of the most commonly used tests in clinical microbiology laboratories for screening fecal cultures and isolating pathogens such as Salmonella spp. and Shigella spp.