Competitive ELISA Protocol and Animation


Enzyme-linked immunosorbent assay (ELISA) is a widely used technique for detecting and quantifying specific molecules, such as proteins, hormones, or antibodies, in a sample. ELISA is based on the principle of antigen-antibody binding and enzyme-mediated signal amplification.

There are different types of ELISA, depending on the format of antigen and antibody immobilization and detection. One of them is Competitive ELISA, which is a technique used for the estimation of antibodies present in a specimen, such as serum, plasma, saliva, or urine.

Competitive ELISA is especially useful for measuring low concentrations of antibodies or antigens that have only one epitope (the part of the antigen that is recognized by the antibody). Competitive ELISA can also overcome some of the limitations of other ELISA formats, such as interference from complex samples or cross-reactivity of antibodies.

In this blog article, we will explain the principle and protocol of Competitive ELISA, and show you an animation that illustrates the process step by step. We will also discuss the advantages and considerations of using Competitive ELISA for your research or diagnostic applications.