Column Chromatography- Definition, Principle, Parts, Steps, Uses


Column chromatography is a technique in which the substances to be separated are introduced onto the top of a column packed with an adsorbent, passed through the column at different rates that depend on the affinity of each substance for the adsorbent and for the solvent or solvent mixture, and are usually collected in solution as they pass from the column at different times. It is a solid-liquid technique in which the stationary phase is a solid and the mobile phase is a liquid or gas. It was developed by the American chemist D.T Day in 1900 while M.S. Tswett, the Polish botanist, 1906 used adsorption columns in his investigations of plant pigments.