Calcofluor White Staining- Principle, Procedure, Results, Applications


Calcofluor white staining is a technique that uses a fluorescent dye to stain the cell walls of fungi, plants, and algae. It is based on the ability of calcofluor white (CFW) to bind to polysaccharides such as chitin and cellulose, which are present in the cell walls of these organisms. When exposed to ultraviolet (UV) light or blue-violet light, CFW emits a bright green fluorescence that can be observed under a fluorescence microscope.