Bile Esculin Test- Principle, procedure, results, uses, limitations


The bile esculin test is a biochemical test that is used to identify and differentiate certain bacteria based on their ability to hydrolyze esculin in the presence of bile. Esculin is a compound that consists of glucose and hydroxycoumarin, which are linked by an ester bond. Hydroxycoumarin is a fluorescent molecule that can be detected by its loss of fluorescence when it is broken down. Bile is a digestive fluid that contains bile salts, which inhibit the growth of most Gram-positive bacteria except for some streptococci and enterococci.