Bial’s Test- Definition, Principle, Procedure, Result, Uses


Bial’s test is a chemical test that is used to detect and differentiate pentoses and pentosans from other carbohydrates. Pentoses are monosaccharides that have five carbon atoms, such as ribose and xylose. Pentosans are polysaccharides that consist of pentose units linked by glycosidic bonds, such as xylan and arabinan. Bial’s test is based on the formation of a blue-green complex when pentoses or pentosans react with orcinol and ferric chloride in acidic conditions. The test can also be used to detect and quantify RNA, which is a nucleic acid that contains ribose as its sugar component. Bial’s test is named after the Polish chemist Napoleon Bial (1857-1917), who developed it in 1892.