Anthrax bacilli vs Anthracoid bacilli- 25 Differences
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Anthrax and anthracoid bacilli are two types of bacteria that belong to the genus Bacillus. They are gram-positive, rod-shaped, spore-forming, and aerobic organisms found in soil and animal products. However, they have different characteristics and pathogenicity that distinguish them.
Anthrax bacilli, also known as Bacillus anthracis, are the causative agents of anthrax, a severe and potentially fatal disease that affects humans and animals. Anthrax bacilli can form endospores highly resistant to heat, chemicals, and desiccation. These endospores can survive for long periods in the environment and can be inhaled, ingested, or inoculated through skin wounds. Depending on the route of infection, anthrax bacilli can cause different forms of disease, such as:
- Cutaneous anthrax: The most common and least severe form of anthrax, characterized by a painless black ulcer (eschar) at the site of infection, accompanied by fever, headache, and lymphadenopathy.
- Gastrointestinal anthrax is a rare but more severe form of anthrax caused by ingesting contaminated food or water. It manifests as abdominal pain, nausea, vomiting, diarrhea, and bleeding.
- Inhalational anthrax: The most lethal form of anthrax, caused by inhalation of aerosolized endospores. It presents flu-like symptoms followed by severe respiratory distress, shock, and death.
- Anthrax meningitis: A complication of any form of anthrax involving the spread of the bacteria to the central nervous system. It results in severe headaches, neck stiffness, confusion, seizures, and coma.
Anthrax bacilli can be diagnosed by culture, microscopy, or molecular methods. They can be treated with antibiotics such as penicillin, doxycycline, or ciprofloxacin. However, early diagnosis and treatment are essential to prevent mortality. Anthrax can also be controlled by vaccination and proper handling of animal products.
Anthracoid bacilli are a group of bacteria that resemble anthrax bacilli in morphology and staining properties. They include Bacillus subtilis, Bacillus cereus, Bacillus licheniformis, and Bacillus megaterium. They are generally considered non-pathogenic or opportunistic pathogens that rarely cause human disease. However, they differ from anthrax bacilli in many ways, as follows:
- Anthracoid bacilli do not form capsules around their cells, unlike anthrax bacilli, which produce a thick capsule composed of poly-D-glutamic acid.
- Anthracoid bacilli do not produce lecithinase (phospholipase C), an enzyme that degrades lecithin in cell membranes and causes hemolysis on blood agar plates. Anthrax bacilli multiply lecithinase, which gives them a characteristic halo effect on blood agar plates.
- Anthracoid bacilli have different biochemical reactions and growth requirements than anthrax bacilli. For example, anthracoid bacilli can grow on MacConkey agar (a selective medium for gram-negative bacteria), while anthrax bacilli cannot.
- Anthracoid bacilli have different antigenic structures than anthrax bacilli. For example, anthracoid bacilli do not have the protective antigen (PA), lethal factor (LF), and edema factor (EF) components that make up the anthrax toxin.
- Anthracoid bacilli have different virulence factors than anthrax bacilli. For example, Bacillus cereus produces enterotoxins that cause food poisoning symptoms such as diarrhea and vomiting.
Anthracoid bacilli can be distinguished from anthrax bacilli by various laboratory tests such as capsule staining, lecithinase, motility, and serological tests. They can also be identified by molecular methods such as PCR or sequencing. Anthracoid bacilli are usually susceptible to antibiotics and do not require specific treatment unless they cause infection.
In conclusion, anthrax and anthracoid bacilli are two types of bacteria that share some similarities but differ in their morphology, physiology, and pathogenicity. Various laboratory methods can differentiate them and should be cautiously handled to prevent misdiagnosis or contamination.
Anthrax bacilli and anthracoid bacilli are gram-positive, aerobic, spore-forming bacteria belonging to the genus Bacillus. They have some morphological and cultural similarities, such as producing dry, wrinkled colonies and appearing as chains of spore-bearing bacilli in smears. However, they differ from each other in many ways, as summarized in the table below :
| S.N. | Character | Anthrax bacilli | Anthracoid bacilli |
| --- | --- | --- | --- |
| 1. | Known as | Bacillus anthracis | B. anthracis similis, B. pseudo-anthracis or "anthrax-like" bacilli and Pseudoanthrax |
| 2. | Shape (Chain Length) | In long chains | In short chains |
| 3. | Position of spores | Central, do not bulge the bacilli | Central, subterminal or terminal, may bulge the bacilli |
| 4. | Capsule | Present | Absent |
| 5. | Motility | Non-motile | Motile |
| 6. | Under lower power microscope | Medusa head colony seen | Not seen |
| 7. | Oxygen requirement | Strict aerobe | Aerobic or facultative anaerobic |
| 8. | Growth at 45°C | No Growth | Growth usually seen |
| 9. | Blood Agar | No hemolysis (or weak) | Hemolytic colonies (usually well marked) |
| 10. | Turbidity | No Turbidity in broth | Turbidity seen usually |
| 11. | Solid medium with penicillin (10 units/ml) | No Growth [^25
Grow Usually |
| 12. | Gelatin stab agar[^4 ^]| Inverted fir tree appearance[^3 ^]| Absent |
| 13. | In nutrient broth[^4 ^]| Fluffy Cotton wool without pellicle[^3 ^]| Turbidity and pellicle formation but no fluffy Cotton wool |
| 14. | Salicin Fermentation[^4 ^]| Negative[^3 ^]| Usually Positive |
| 15. | Rate of gelatin liquefaction[^4 ^]| Slow[^3 ^]| Rapid |
| 16. | Lecithinase activity on egg yolk agar[^4 ^]| — weak[^3 ^]| + marked |
| 17. | Reduction of methylene blue in milk[^4 ^]| Reduce methylene blue slowly[^3 ^]| Rapidly reduce methylene blue |
| 18. | Chloral Hydrate[^4 ^]| Growth inhibited by Chloral Hydrate[^3 ^]| Not Inhibited |
| 19. | Susceptibility to Gamma Phage[^4 ^]| Susceptible[^3 ^]| Not susceptible |
| 20. | Penicillin sensitivity (10 unit disc)[4 ]| Susceptible[^3 ^]| Resistant |
| 21. | Pathogens[^2 ^]| Pathogenic[^3 ^]| Except for B. cereus, most are non-pathogenic or opportunistic pathogens with low virulence.[2 ]|
| 22. | Diseases caused[^2 ^]| ■ Cutaneous anthrax ■ Gastrointestinal anthrax ■ Inhalational anthrax ■ Anthrax meningitis.[2 ]| ■ Bacillus cereus is the most important pathogen causing GI, ocular, and catheter-related infections.[2 ] ■ Bacillus subtilis may act as an opportunistic pathogen, causing eye infections and septicemia.[2 ] ■ Bacillus licheniformis has also been incriminated in patients with food poisoning.[2 ]|
| 23. | Pathogenicity for mice or guinea pigs[^4 ^]| Pathogenic (death in 24 – 48 hours)[3 ]| No death[3 ]|
| 24. | Contaminants[^2 ^]| Not a common contaminant.[2 ]| Common contaminants in laboratory cultures.[2 ]|
| 25. Organism (s)[2 ]| Bacillus anthracis[2 ]| B. cereus, B. subtilis, B. licheniformis, B. stearothermophilus etc.[2 ]|
As you can see, anthrax bacilli and anthracoid bacilli have many differences that can help distinguish them in laboratory diagnosis and clinical settings.
Anthrax and Anthracoid bacilli are Gram-positive, aerobic, spore-forming bacteria belonging to the genus Bacillus. They have some similarities in their morphology and culture, such as producing dry, wrinkled colonies and appearing as chains of spore-bearing bacilli in the smear. However, they differ from each other in many ways, as shown in the table below :
| S.N. | Character | Anthrax bacilli | Anthracoid bacilli |
| --- | --- | --- | --- |
| 1. | Known as | Bacillus anthracis | B. anthracis similis, B. pseudo-anthracis or "anthrax-like" bacilli and pseudo-anthrax. |
| 2. | Shape (Chain Length) | In long chains | In short chains |
| 3. | Position of spores | Central, do not bulge the bacilli. | Central, subterminal, or terminal may bulge the bacilli. |
| 4. | Capsule | Present | Absent |
| 5. | Motility | Non-motile | Motile |
| 6. | Under lower power microscope | Medusa head colony seen | Not seen |
| 7. | Oxygen requirement | Strict aerobe | Aerobic or facultative anaerobic |
| 8. | Growth at 45°C | No Growth | Growth usually seen |
| 9. | Blood Agar | No hemolysis (or weak) | Hemolytic colonies (usually well marked) |
| 10. | Turbidity | No Turbidity in broth | Turbidity seen usually |
| 11. | Solid medium with penicillin String of pearls appearance | No growth |
| 12. | Gelatin stab agar Inverted fir tree appearance Absent
| 13. In nutrient broth, Fluffy Cotton wool without pellicle Turbidity and pellicle formation but no fluffy Cotton wool
| 14. Salicin Fermentation Negative Usually Positive
| 15. Rate of gelatin liquefaction Slow Rapid
| 16. Lecithinase activity on egg yolk agar — weak + marked
| 17. Reduction of methylene blue in milk Reduce methylene blue slowly Rapidly reduce methylene blue
| 18. Chloral Hydrate Growth inhibited by Chloral Hydrate Not Inhibited
| 19. Susceptibility to Gamma Phage Susceptible, Not susceptible
| 20. Penicillin sensitivity 10 unit disc Susceptible Resistant
| 21. Pathogens Pathogenic Except for B. cereus, most are non-pathogenic or opportunistic pathogens with low virulence.
| 22. Diseases caused ■ Cutaneous anthrax ■ Gastrointestinal anthrax ■ Inhalational anthrax ■ Anthrax meningitis. ■ Bacillus cereus is the most important pathogen causing GI, ocular, and catheter-related infections. ■ Bacillus subtilis may act as an opportunistic pathogen, causing eye infections and septicemia. ■ Bacillus licheniformis has also been incriminated in patients with food poisoning.
| 23. Pathogenicity for mice or guinea pigs Pathogenic (death in 24 – 48 hours) No death
| 24. Contaminants Not a common contaminant Common contaminants in laboratory cultures
| 25 Organism (s) Bacillus anthracis B cereus B subtilis B licheniformis B stearothermophilus etc
As can be seen from the table, Anthrax bacilli and Anthracoid bacilli have many distinctive characteristics that can be used to differentiate them from each other and other Bacillus species.
Another essential difference between anthrax and anthracoid bacilli is the presence or absence of a capsule and lecithinase production. A capsule is a layer of polysaccharides or polypeptides that surrounds some bacteria and protects them from phagocytosis and other host defenses. Lecithinase is an enzyme that hydrolyzes lecithin, a phospholipid found in cell membranes and other biological structures.
Anthrax bacilli have a unique capsule composed of poly-D-glutamic acid, which is not found in other bacteria. This capsule is essential for the virulence of anthrax bacilli, as it inhibits the complement system and prevents opsonization and phagocytosis by macrophages. Anthrax bacilli also produce lecithinase, which is encoded by the plasmid pXO1. Lecithinase contributes to the pathogenesis of anthrax by destroying the cell membranes of host cells and causing tissue necrosis and edema.
Anthracoid bacilli, on the other hand, do not have a capsule or produce lecithinase. This makes them less virulent and more susceptible to host defenses. Anthracoid bacilli can be distinguished from anthrax bacilli by their negative results in the capsule staining test and the lecithinase test. The capsule staining test uses India ink or nigrosin to shade the background while leaving the tablet unstained. The lecithinase test uses egg yolk agar to detect the formation of a white precipitate around the colonies due to lecithin hydrolysis.
The capsule and lecithinase differences between anthrax and anthracoid bacilli are essential for their identification and diagnosis. They also reflect their different modes of infection and pathogenicity in humans and animals.
Anthrax and anthracoid bacilli are gram-positive, spore-forming rods belonging to the genus Bacillus. They share some common characteristics, such as being aerobic or facultatively anaerobic, catalase-positive, and motile. However, they also have many differences that distinguish them from each other. Anthrax bacilli are the causative agents of anthrax, a severe and potentially fatal disease that affects humans and animals. Anthracoid bacilli are a group of environmental and opportunistic bacteria that can cause infections in immunocompromised or injured hosts. The main differences between anthrax and anthracoid bacilli are:
- Anthrax bacilli produce a capsule composed of poly-D-glutamic acid, which protects them from phagocytosis and enhances their virulence. Anthracoid bacilli do not have a pill or create a different type of capsule that is not antiphagocytic.
- Anthrax bacilli produce lecithinase, an enzyme that hydrolyzes lecithin and causes hemolysis and tissue necrosis. Anthracoid bacilli do not have lecithinase or create a different type of lecithinase that is not hemolytic or necrotic.
- Anthrax bacilli are non-hemolytic on blood agar, whereas anthracoid bacilli are usually hemolytic.
- Anthrax bacilli are sensitive to penicillin and other beta-lactam antibiotics, whereas anthracoid bacilli are usually resistant to penicillin and other beta-lactam antibiotics.
- Anthrax bacilli have a characteristic bamboo-shaped appearance under the microscope, whereas anthracoid bacilli have a more uniform appearance.
These differences can help identify and diagnose anthrax and anthracoid bacilli infections. They can also guide the appropriate treatment and prevention of these infections. Anthrax is a severe public health threat that requires prompt recognition and management. Anthracoid bacilli are less virulent but can still cause infections in susceptible hosts. Therefore, both types of bacteria should be handled with caution and care in the laboratory and clinical settings.
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