Acridine Orange Stain- Principle, Procedure and Result Interpretation


The objective of acridine orange stain is to differentiate between nucleic acids, such as DNA and RNA, in biological samples. Nucleic acids are essential molecules that store and transmit genetic information in living organisms. They are composed of nucleotides, which are units of a nitrogenous base, a sugar, and a phosphate group. DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) are the two main types of nucleic acids found in cells. DNA is usually double-stranded and forms a helical structure, while RNA is usually single-stranded and can fold into various shapes. DNA contains the bases adenine (A), thymine (T), cytosine (C), and guanine (G), while RNA contains the bases adenine (A), uracil (U), cytosine (C), and guanine (G).