Acetate Utilization Test- Principle, Procedure, Results, Uses


Acetate utilization test is a biochemical test that determines the ability of an organism to use acetate as the sole source of carbon. Acetate is a simple organic acid that can be metabolized by some bacteria in the presence of inorganic nitrogen. The test is based on the principle that the utilization of acetate by the bacteria results in the production of ammonia, which raises the pH of the medium and changes the color of the pH indicator from green to blue.